Radioimmunoassay of penicilloyl groups in biological fluids
نویسندگان
چکیده
منابع مشابه
Measurement of leukemia inhibitory factor in biological fluids by radioimmunoassay.
Leukemia inhibitory factor (LIF) exhibits multiple biological activities in various tissues, and we have shown that LIF activates POMC gene transcription in response to immune signals. As higher serum levels of LIF have been reported in septicemia, we measured LIF values in biological fluids by RIA. Immunoreactive LIF was detected in 303 of 428 human serum samples. Circulating LIF detection rat...
متن کاملFixation of penicilloyl groups to albumin and appearance of anti-penicilloyl antibodies in penicillin-treated patients.
Penicilloyl groups, which have been connected to penicillin allergy, are derived from penicillin by cleavage of the beta lactam ring and bind covalently to proteins. Fixation of penicilloyl groups was studied in seven patients given large amounts of penicillin. Penicilloyl groups were found essentially on the albumin molecule at sites not accessible to anti-penicilloyl antibodies, except after ...
متن کاملA simple, rapid 125I radioimmunoassay for the detection of barbiturates in biological fluids.
A simple, rapid radioimmunoassay employing 125-I-labeled secobarbital derivative has been developed and has been shown to be capable of detecting at the nanogram level a variety of barbiturates in urine as well as in plasma.
متن کاملDEVELOPMENT OF A RAPID AND SENSITIVE RADIOIMMUNOASSAY FOR MEASUREMENT OF AFLATOXIN B 1 IN BIOLOGICAL SAMPLES
Aflatoxin B I (AFB) isa well known hepatocarcinogen in several animal species and probably a causative agent in human hepatocellular carcinoma. Humans are exposed to AFB by ingesting contaminated food. Aflatoxin contamination encountered in human foods is usually at low levels which is difficult to measure by chromatographic methods. Therefore in the present study we have developed an immun...
متن کاملRadioimmunoassay of dibenzazepines and dibenzcycloheptanodienes in body fluids and tissues.
In an attempt to establish a radioimmunoassay (RIA), imipramine and amitriptyline immunogens were prepared; desmethyl derivatives were converted into hemisuccinates, conjugated with bovine serum albumin and used for rabbit immunization. [3H]Amitriptyline (4.3 TBq/mmol) and [3H]imipramine (2.9 TBq/mmol) were prepared by catalytic dehalogenation or reductive alkylation. Dibenzazepines and dibenzc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)80140-2